《植物生理学报》 2014, 50(10): 1535-1540

三七SE基因启动子的克隆及序列分析

卢荣江, 刘迪秋, 葛锋*, 陈朝银

昆明理工大学生命科学与技术学院, 昆明650500

通信作者：葛锋；E-mail: gefeng@tsinghua.org.cn；Tel: 0871-65920621

摘　要：

鲨烯环氧酶(squalene epoxidase, SE)基因是三七皂苷生物合成途径中的关键基因。本文采用染色体步移(genomic walking)技术, 克隆出三七SE基因上游1 872 bp的启动子片段。生物信息学分析表明, 该启动子除了含有基本的TATA-box和CAAT-box元件外, 还包含多个与细胞分裂素响应、抗病以及糖类和储藏蛋白合成等相关的作用元件。

关键词：三七; 鲨烯环氧酶; 启动子

收稿：2014-05-06   修定：2014-07-25

资助：国家自然科学基金项目(31260070)。

Cloning and Sequence Analysis of Promoter of SE in Panax notoginseng

LU Rong-Jiang, LIU Di-Qiu, GE Feng*, CHEN Chao-Yin

Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China

Corresponding author: GE Feng; E-mail: gefeng@tsinghua.org.cn; Tel: 0871-65920621

Abstract:

The squalene epoxidase (SE) is considered to be a key enzyme involved in Panax notoginseng biosynthesis pathway. A 1 872 bp promoter fragment was cloned from P. notoginseng using genomic walking technique. The bioinformatics analyses showed that the fragment contained the conserved promoter sequence, such as TATA-box, CAAT-box. Furthermore, it contained several elements related to cytokinin response, disease-related, the synthesis of carbohydrates and proteins.

Key words: Panax notoginseng; squalene epoxidase; promoter